Levchenko lab

The mission of our lab is to further our understanding of how cells communicate with each other, interpret cues from the environment and neighboring cells, and ultimately process this information through signalling pathways to direct cell form and function. Although we use a variety of experimental methodologies, almost all of our work incorporates studying the above phenomena on the level of single cells using fluorescence microscopy. Moreover, at heart, we are a computationally oriented laboratory, and seek to take quantitative fluorescent measurements for most of our work.

Through many years of experience, we have found that having a stable light source is an invaluable part of making fluorescence measurements both accurate and repeatable. Here, we are happy to point out the X-Cite® exacte has been of great help in achieving these measurements, both using live cell techniques with intensity and FRET based probes, and using immunostaining techniques. The Closed-Loop Feedback control found in the exacte is an innovative technology allowing the stability needed for our measurements while also providing abundant light source intensity. In addition, the long lifetime of the exacte bulb allows us to conduct our experiments without being interrupted by monthly bulb changes.

Website: https://jshare.johnshopkins.edu/alevche1/web/ | Contact: alev@jhu.edu