FRET (Fluorescence or Förster Resonance Energy Transfer) is a quantum mechanical process involving the non-radiative transfer of energy between fluorophores over a small distance (1-10 nm). If the molecules are close enough, the donor fluorophore transfers its energy to the acceptor fluorophore. Monitoring the ratio of the fluorophore emissions provides valuable information to researchers about the spatial localization of the proteins being studied.
The following article was published in Bioscience Technology, June 2010 issue, Pg 11.
www.BioscienceTechnology.com
FRET (Förster Resonance Energy Transfer) microscopy imaging is a widely used technique to determine protein interactions inside living cells. It can answer several questions about the extent of protein colocalization, dimerization, folding, molecular motors and transduction pathways. FRET is a quantum mechanical process involving the radiation-less transfer of energy between fluorophores over a small distance (1-10 nm). If the molecules are close enough, the donor fluorophore transfers its energy to the acceptor fluorophore. Monitoring the ratio of the fluorophore emissions provides valuable information to researchers about the spatial localization of the proteins being studied.
X-Cite® customers have found that using the X-Cite® exacte system reduces the need to normalize the ratio of the fluorescence signals. The X-Cite® exacte system provides fluorescence illumination that is more stable than the traditional arc-lamp and offers greater control over variables which cause undesirable artifacts in a FRET set up. Researchers can modulate the light output to optimize imaging conditions and minimize photobleaching, while having a stable fluorescence light source for imaging over long periods of time (10-24 hours time-lapse FRET experiments).
The X-Cite® exacte system provides unparalleled stability through its DC powered lamp and Closed-Loop Feedback mechanism, ensuring stable fluorescence illumination over the course of the experiment - whether it lasts for milliseconds or days. The Intelli-Lamp® technology ensures worry-free operation with pre-aligned, long-life lamps.
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